BME Seminar: Label-free optical metabolic imaging for biomanufacturing
October 18 @ 12:00 pm - 1:00 pm
Melissa Skala, PhD; Investigator at Morgridge Institute; Professor of Biomedical Engineering; University of Wisconsin-Madison
T cell and stem cell manufacturing are two major components of the cell therapy market. However, cost-effective and time-efficient technologies are needed to monitor the quality of cells during biomanufacturing. We have developed a non-invasive and label-free live cell imaging platform to monitor T cell function and predict the efficiency of stem cell differentiation into cardiomyocytes. Fluorescence lifetime imaging of the metabolic co-enzymes NAD(P)H and FAD was coupled with single cell segmentation and prospective classification algorithms. This approach identified activated T cells with high accuracy and predicted stem cell differentiation efficiency early (day 1) with high accuracy. Autofluorescence imaging of single cells also quantified changes in metabolism during cardiomyocyte maturation. This non-invasive and label-free method could be used monitor cell manufacturing for T cell and stem cell therapies, reducing the cost and time to produce these therapies.